Gene isolation using degenerate primers targeting protein motif: A laboratory exercise
نویسندگان
چکیده
منابع مشابه
Designing Of Degenerate Primers-Based Polymerase Chain Reaction (PCR) For Amplification Of WD40 Repeat-Containing Proteins Using Local Allignment Search Method
Degenerate primers-based polymerase chain reaction (PCR) are commonly used for isolation of unidentified gene sequences in related organisms. For designing the degenerate primers, we propose the use of local alignment search method for searching the conserved regions long enough to design an acceptable primer pair. To test this method, a WD40 repeat-containing domain protein from Beauveria bass...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2017
ISSN: 1470-8175
DOI: 10.1002/bmb.21089